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biotinylated goat anti human il8  (R&D Systems)


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    R&D Systems biotinylated goat anti human il8
    <t>IL8</t> protein production from Holstein and Angus fibroblasts. a IL8 production by dermal fibroblasts isolated from 19-month old Holstein ( n = 5) and Angus ( n = 12) heifers and challenged for 24 h with LPS (100ng/ml) or IL1B (1ng/ml). b Eight cultures ( n = 4/breed) were randomly chosen for RNA-seq and MIRA-seq analysis and levels of IL8 protein following a second 24 h challenge with either LPS (100ng/ml) or IL1B (1ng/ml) on these 8 cultures are shown. There was no detectable IL8 production from un-treated cultures of both breeds. Values are mean ± SEM. * indicates P < 0.05, ** indicates P < 0.01
    Biotinylated Goat Anti Human Il8, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+goat+anti+human+il8/pmc04806443-297-16-20?v=R%26D+Systems
    Average 94 stars, based on 76 article reviews
    biotinylated goat anti human il8 - by Bioz Stars, 2026-08
    94/100 stars

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    1) Product Images from "Differential responsiveness of Holstein and Angus dermal fibroblasts to LPS challenge occurs without major differences in the methylome"

    Article Title: Differential responsiveness of Holstein and Angus dermal fibroblasts to LPS challenge occurs without major differences in the methylome

    Journal: BMC Genomics

    doi: 10.1186/s12864-016-2565-x

    IL8 protein production from Holstein and Angus fibroblasts. a IL8 production by dermal fibroblasts isolated from 19-month old Holstein ( n = 5) and Angus ( n = 12) heifers and challenged for 24 h with LPS (100ng/ml) or IL1B (1ng/ml). b Eight cultures ( n = 4/breed) were randomly chosen for RNA-seq and MIRA-seq analysis and levels of IL8 protein following a second 24 h challenge with either LPS (100ng/ml) or IL1B (1ng/ml) on these 8 cultures are shown. There was no detectable IL8 production from un-treated cultures of both breeds. Values are mean ± SEM. * indicates P < 0.05, ** indicates P < 0.01
    Figure Legend Snippet: IL8 protein production from Holstein and Angus fibroblasts. a IL8 production by dermal fibroblasts isolated from 19-month old Holstein ( n = 5) and Angus ( n = 12) heifers and challenged for 24 h with LPS (100ng/ml) or IL1B (1ng/ml). b Eight cultures ( n = 4/breed) were randomly chosen for RNA-seq and MIRA-seq analysis and levels of IL8 protein following a second 24 h challenge with either LPS (100ng/ml) or IL1B (1ng/ml) on these 8 cultures are shown. There was no detectable IL8 production from un-treated cultures of both breeds. Values are mean ± SEM. * indicates P < 0.05, ** indicates P < 0.01

    Techniques Used: Isolation, RNA Sequencing

    Average response to LPS from combined Holstein and Angus cultures measured by increases in expression of immune-associated genes compared to hour 0 post-LPS
    Figure Legend Snippet: Average response to LPS from combined Holstein and Angus cultures measured by increases in expression of immune-associated genes compared to hour 0 post-LPS

    Techniques Used: Expressing, Activation Assay, Ubiquitin Proteomics, Virus

    Differential gene expression (fold change) of immune-associated genes at hours 0, 2, and 8 post-LPS treatment of fibroblasts collected from Holstein animals as compared to fibroblasts collected from Angus animals
    Figure Legend Snippet: Differential gene expression (fold change) of immune-associated genes at hours 0, 2, and 8 post-LPS treatment of fibroblasts collected from Holstein animals as compared to fibroblasts collected from Angus animals

    Techniques Used: Gene Expression, DNA Methylation Assay

    Selected gene expression by Holstein and Angus fibroblasts in response to LPS. Differences in expression of TLR4 ( a ) IL8 ( b ) TNF ( c ) CCL20 ( d ) and CCL5 ( e ) between Holstein and Angus fibroblast cultures at hours 0, 2, and 8 post-LPS exposure were determined by RT-qPCR. Values are expressed as delta Ct, or the difference in cycles to threshold (Ct) between the gene of interest and the endogenous gene control beta-actin ( ACTB) . Fold differences (2 -ΔΔCt ) in expression between Holstein and Angus cultures are indicated in parentheses. All values are mean ± SEM. ( n = 4/group). * P < 0.05 and ** P < 0.01 ( t -test)
    Figure Legend Snippet: Selected gene expression by Holstein and Angus fibroblasts in response to LPS. Differences in expression of TLR4 ( a ) IL8 ( b ) TNF ( c ) CCL20 ( d ) and CCL5 ( e ) between Holstein and Angus fibroblast cultures at hours 0, 2, and 8 post-LPS exposure were determined by RT-qPCR. Values are expressed as delta Ct, or the difference in cycles to threshold (Ct) between the gene of interest and the endogenous gene control beta-actin ( ACTB) . Fold differences (2 -ΔΔCt ) in expression between Holstein and Angus cultures are indicated in parentheses. All values are mean ± SEM. ( n = 4/group). * P < 0.05 and ** P < 0.01 ( t -test)

    Techniques Used: Gene Expression, Expressing, Quantitative RT-PCR, Control

    RT-qPCR primer pairs used for amplification of target genes
    Figure Legend Snippet: RT-qPCR primer pairs used for amplification of target genes

    Techniques Used: Amplification, Sequencing



    Similar Products

    biotinylated goat anti human il8  (R&D Systems)
    94
    R&D Systems biotinylated goat anti human il8
    <t>IL8</t> protein production from Holstein and Angus fibroblasts. a IL8 production by dermal fibroblasts isolated from 19-month old Holstein ( n = 5) and Angus ( n = 12) heifers and challenged for 24 h with LPS (100ng/ml) or IL1B (1ng/ml). b Eight cultures ( n = 4/breed) were randomly chosen for RNA-seq and MIRA-seq analysis and levels of IL8 protein following a second 24 h challenge with either LPS (100ng/ml) or IL1B (1ng/ml) on these 8 cultures are shown. There was no detectable IL8 production from un-treated cultures of both breeds. Values are mean ± SEM. * indicates P < 0.05, ** indicates P < 0.01
    Biotinylated Goat Anti Human Il8, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+goat+anti+human+il8/pmc04806443-297-16-20?v=R%26D+Systems
    Average 94 stars, based on 1 article reviews
    biotinylated goat anti human il8 - by Bioz Stars, 2026-08
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    IL8 protein production from Holstein and Angus fibroblasts. a IL8 production by dermal fibroblasts isolated from 19-month old Holstein ( n = 5) and Angus ( n = 12) heifers and challenged for 24 h with LPS (100ng/ml) or IL1B (1ng/ml). b Eight cultures ( n = 4/breed) were randomly chosen for RNA-seq and MIRA-seq analysis and levels of IL8 protein following a second 24 h challenge with either LPS (100ng/ml) or IL1B (1ng/ml) on these 8 cultures are shown. There was no detectable IL8 production from un-treated cultures of both breeds. Values are mean ± SEM. * indicates P < 0.05, ** indicates P < 0.01

    Journal: BMC Genomics

    Article Title: Differential responsiveness of Holstein and Angus dermal fibroblasts to LPS challenge occurs without major differences in the methylome

    doi: 10.1186/s12864-016-2565-x

    Figure Lengend Snippet: IL8 protein production from Holstein and Angus fibroblasts. a IL8 production by dermal fibroblasts isolated from 19-month old Holstein ( n = 5) and Angus ( n = 12) heifers and challenged for 24 h with LPS (100ng/ml) or IL1B (1ng/ml). b Eight cultures ( n = 4/breed) were randomly chosen for RNA-seq and MIRA-seq analysis and levels of IL8 protein following a second 24 h challenge with either LPS (100ng/ml) or IL1B (1ng/ml) on these 8 cultures are shown. There was no detectable IL8 production from un-treated cultures of both breeds. Values are mean ± SEM. * indicates P < 0.05, ** indicates P < 0.01

    Article Snippet: Mouse anti-bovine (clone 170.13, gifted by Samuel Maheswaren, University of Minnesota, St. Paul, MN) and a biotinylated goat anti-human IL8 (R&D Systems Inc., Minneapolis, MN) were used as capture and detection antibodies, respectively.

    Techniques: Isolation, RNA Sequencing

    Average response to LPS from combined Holstein and Angus cultures measured by increases in expression of immune-associated genes compared to hour 0 post-LPS

    Journal: BMC Genomics

    Article Title: Differential responsiveness of Holstein and Angus dermal fibroblasts to LPS challenge occurs without major differences in the methylome

    doi: 10.1186/s12864-016-2565-x

    Figure Lengend Snippet: Average response to LPS from combined Holstein and Angus cultures measured by increases in expression of immune-associated genes compared to hour 0 post-LPS

    Article Snippet: Mouse anti-bovine (clone 170.13, gifted by Samuel Maheswaren, University of Minnesota, St. Paul, MN) and a biotinylated goat anti-human IL8 (R&D Systems Inc., Minneapolis, MN) were used as capture and detection antibodies, respectively.

    Techniques: Expressing, Activation Assay, Ubiquitin Proteomics, Virus

    Differential gene expression (fold change) of immune-associated genes at hours 0, 2, and 8 post-LPS treatment of fibroblasts collected from Holstein animals as compared to fibroblasts collected from Angus animals

    Journal: BMC Genomics

    Article Title: Differential responsiveness of Holstein and Angus dermal fibroblasts to LPS challenge occurs without major differences in the methylome

    doi: 10.1186/s12864-016-2565-x

    Figure Lengend Snippet: Differential gene expression (fold change) of immune-associated genes at hours 0, 2, and 8 post-LPS treatment of fibroblasts collected from Holstein animals as compared to fibroblasts collected from Angus animals

    Article Snippet: Mouse anti-bovine (clone 170.13, gifted by Samuel Maheswaren, University of Minnesota, St. Paul, MN) and a biotinylated goat anti-human IL8 (R&D Systems Inc., Minneapolis, MN) were used as capture and detection antibodies, respectively.

    Techniques: Gene Expression, DNA Methylation Assay

    Selected gene expression by Holstein and Angus fibroblasts in response to LPS. Differences in expression of TLR4 ( a ) IL8 ( b ) TNF ( c ) CCL20 ( d ) and CCL5 ( e ) between Holstein and Angus fibroblast cultures at hours 0, 2, and 8 post-LPS exposure were determined by RT-qPCR. Values are expressed as delta Ct, or the difference in cycles to threshold (Ct) between the gene of interest and the endogenous gene control beta-actin ( ACTB) . Fold differences (2 -ΔΔCt ) in expression between Holstein and Angus cultures are indicated in parentheses. All values are mean ± SEM. ( n = 4/group). * P < 0.05 and ** P < 0.01 ( t -test)

    Journal: BMC Genomics

    Article Title: Differential responsiveness of Holstein and Angus dermal fibroblasts to LPS challenge occurs without major differences in the methylome

    doi: 10.1186/s12864-016-2565-x

    Figure Lengend Snippet: Selected gene expression by Holstein and Angus fibroblasts in response to LPS. Differences in expression of TLR4 ( a ) IL8 ( b ) TNF ( c ) CCL20 ( d ) and CCL5 ( e ) between Holstein and Angus fibroblast cultures at hours 0, 2, and 8 post-LPS exposure were determined by RT-qPCR. Values are expressed as delta Ct, or the difference in cycles to threshold (Ct) between the gene of interest and the endogenous gene control beta-actin ( ACTB) . Fold differences (2 -ΔΔCt ) in expression between Holstein and Angus cultures are indicated in parentheses. All values are mean ± SEM. ( n = 4/group). * P < 0.05 and ** P < 0.01 ( t -test)

    Article Snippet: Mouse anti-bovine (clone 170.13, gifted by Samuel Maheswaren, University of Minnesota, St. Paul, MN) and a biotinylated goat anti-human IL8 (R&D Systems Inc., Minneapolis, MN) were used as capture and detection antibodies, respectively.

    Techniques: Gene Expression, Expressing, Quantitative RT-PCR, Control

    RT-qPCR primer pairs used for amplification of target genes

    Journal: BMC Genomics

    Article Title: Differential responsiveness of Holstein and Angus dermal fibroblasts to LPS challenge occurs without major differences in the methylome

    doi: 10.1186/s12864-016-2565-x

    Figure Lengend Snippet: RT-qPCR primer pairs used for amplification of target genes

    Article Snippet: Mouse anti-bovine (clone 170.13, gifted by Samuel Maheswaren, University of Minnesota, St. Paul, MN) and a biotinylated goat anti-human IL8 (R&D Systems Inc., Minneapolis, MN) were used as capture and detection antibodies, respectively.

    Techniques: Amplification, Sequencing